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Brigitta Globke: Digital Clinician Scientist
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Dr. Brigitta Globke successfully applied for participation in the BIH Charite Digital Clinician Scientist Program.

The aim of the project is the development and evaluation of an augmented reality assist system for intraoperative photoplethysmographic control of perfusion. The project is carried out in collaboration with Benjamin Kossack, Fraunhofer | Heinrich Hertz Institute Computer Vision and Graphics.

Charité and BIH are jointly organizing the new "Digital Clinician Scientist Program" (D-CSP). The program is primarily aimed at physicians who are already working on innovative research projects to meet the technological challenges of data-driven medicine during their specialist training. The German Research Foundation (DFG) is funding the project for an initial period of three years.

The BIH Charité Digital Clinician Scientist Program will provide a new career path for the creators of digital change in medicine and will expand the successful Germany-wide model of the BIH Charité Clinician Scientist Program. In addition to the three-year individual funding, which is based on protected time for research, the focus is on modules for the acquisition of scientific skills (Big Data, bioinformatics or artificial intelligence) as well as mandatory mentoring. For the new program, various experts* from the Charité, the BIH, the Max Delbrück Center for Molecular Medicine (MDC), the Berlin Institute for Medical Systems Biology (BIMSB), the Einstein Center for Digital Future, and the Bernstein Center for Computational Neuroscience will be involved in the design of the D-CSP and in the recruitment and supervision of program participants.
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Our manuscript "Depletion of donor dendritic cells ameliorates immunogenicity of both skin and hind limb transplants" has been accepted for publication in Frontiers in Immunology, section Alloimmunity and Transplantation. Authors are Muhammad Imtiaz Ashraf, Joerg Mengwasser, Anja Reutzel-Selke, Dietrich Polenz, Kirsten Führer, Steffen Lippert, Peter Tang, Edward Michaelis, Rusan Catar, Johann Pratschke, Christian Witzel, Igor M. Sauer, Stefan G. Tullius, and Barbara Kern.

Acute cellular rejection remains a significant obstacle affecting successful outcomes of organ transplantation including vascularized composite tissue allografts (VCA). Donor antigen presenting cells (APC), particularly dendritic cells (DC), orchestrate early alloimmune responses by activating recipient effector T cells. Employing a targeted approach, we investigated the impact of donor-derived conventional DC (cDC) and APC on the immunogenicity of skin and skin-containing VCA grafts, using mouse models of skin and hind limb transplantation.
By post-transplantation day 6, skin grafts demonstrated severe rejections, characterized by predominance of recipient CD4 T cells. In contrast, hind limb grafts showed moderate rejection, primarily infiltrated by CD8 T cells. While donor depletion of cDC and APC reduced frequencies, maturation, and activation of DC in all analysed tissues of skin transplant recipients, reduction in DC activities was only observed in the spleen of hind limb recipients. Donor cDC and APC depletion did not impact all lymphocyte compartments but significantly affected CD8 T cells and activated CD4 T in lymph nodes of skin recipients. Moreover, both donor APC and cDC depletion attenuated the Th17 immune response, evident by significantly reduced Th17 (CD4+IL-17+) cells in the spleen of skin recipients and reduced levels of IL-17E and lymphotoxin-α in the serum samples of both skin and hind limb recipients. In conclusion, our findings underscore the highly immunogenic nature of skin component in VCA. The depletion of donor APC and cDC mitigates the immunogenicity of skin grafts while exerting minimal impact on VCA.

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