Dr. rer. medic. Moritz Queisner received his doctorate certificate today (magna cum laude)! This is in recognition of his work in the field of extended reality technology in visceral surgery. His thesis is entitled XR in surgery – spatial end embodied computing in digital surgery: technology, application, design.
Joseph Gassner successfully defended his thesis summa cum laude ! His work focused on the optimisation of normothermic ex vivo machine perfusion of rat liver by dialysis and Kupffer cell inhibition with glycine.
Today, Simon Moosburner successfully defended his doctoral thesis entitled "Erweiterung der Spenderpopulation
bei Lebertransplantation: Klinischer Bedarf und Entwicklung eines Kleintier-Lebermaschinenperfusionssystems (Expanding the donor pool for liver transplantation: clinical need and development of small animal liver perfusion system)" summa cum laude !
Martin Kluge is wearing excellent suits and successfully defended his doctoral thesis magna cum laude! He examined the effects of the magnetic field of magnetic resonance imaging (MRI) systems on cells labeled with micrometer-sized iron oxide particles.
Today, Antje Butter successfully defended her doctoral thesis magna cum laude! Congratulations!
Antje was involved in basic research with respect to liver decellularization and recellularization. A proprietary, customized bioreactor was established to repopulate decellularized rat livers with primary rat hepatocytes via the hepatic artery and to subsequently evaluate graft morphology and function during 7 days of ex vivo perfusion. More information via this link.
Today, Jan Schröder successfully defended his thesis (summa cum laude) entitled "Vergleichende in vivo und in vitro Analysen im Rahmen der Etablierung der kombinierten Leber- und Leberzelltransplantation im Rattenmodell" !
Today, Carolin Langer successfully defended her thesis entitled Evaluierung eines Silizium-basierten Eisenoxidpartikels als intrazelluläres Magnetresonanzkontrastmittel für die Leberzelltransplantation „magna cum laude“.
Cellular therapies require methods for noninvasive visualization of transplanted cells. Micron-sized iron oxide particles (MPIOs) generate a strong contrast in magnetic resonance imaging (MRI) and are therefore ideally suited as an intracellular contrast agent to image cells under clinical conditions. However, MPIOs were previously not applicable for clinical use. her thesis focussed on the development and evaluation of silica-based micron-sized iron oxide particles (sMPIOs) with a functionalizable particle surface. Labeling was stable and had no adverse effects on labeled cells. Silica is a biocompatible material that has been approved for clinical use. sMPIOs could therefore be suitable for future clinical applications in cellular MRI, especially in settings that require strong cellular contrast. Moreover, the particle surface provides the opportunity to create multifunctional particles for targeted delivery and diagnostics.
Today, Henriette Riedel successfully defended her thesis entitled Etablierung der kombinierten Transplantation syngener Hepatozyten mit allogenem Lebertransplantat im Rattenmodell zur Untersuchung der Toleranzinduktion „magna cum laude“.
Today, Nils Billecke successfully defended his doctoral thesis magna cum laude. The title of his presentation was „Bioreaktorsystem zur videomikroskopischen Langzeituntersuchung von Zellen in Mono- und Kokultur“.
Today, Rosa Schmuck received the 2012 Charité Robert-Koch-Prize for her doctoral thesis entitled „Genotypic and phenotypic characterization of side population of gastric cancer cell lines“ (group of Prof. C. Röcken).
The Side Population (SP) of tumor cell lines shares characteristics with tumor stem cells. In this study we phenotypically and genotypically characterized the SP of gastric cancer cell lines. SPs were obtained from MKN45- and AGS-gastric cancer cells using Hoechst 33342 staining and fluorescence-activated cell sorting (FACS). SP cells were subsequently studied morphologically (cytology, immunocytochemistry), on the transcriptional level (gene array) and in cell culture (recultivation assays). Genes differentially expressed in the SP cells were evaluated by immunohistochemistry in tissue from gastric cancer patients. SP cells were smaller and rounder then Non-SP cells. SP cells self-renewed in re-cultivation experiments and differentiated into SP- and Non-SP cells. Re-cultivated SP- and Non-SP cells showed distinct phenotypes in culture regarding cell shape and colony-formation. SP cells had increased levels of the stem cell markers CD133 and Musashi1. Transcriptional analyses demonstrated that SP cells express genes that encode for stem cell properties like FZD7, HEY1, SMO and ADAM17. Finally she found ADAM17 and FZD7 to be differentially expressed in human gastric cancer, with FZD7- positive intestinal type cancers showing a significant shorter patient survival. In conclusion human gastric cancer cell lines enclose a phenotypically and genotypically distinct cell population with tumor stem cell features. Phenotypical characteristics of this distinct cell population are also present in gastric cancer tissue and seem to correlate with patient survival.
Haluk Morgül successfully defended his medical doctoral thesis "magna cum laude". After years of extremely fruitful research in the field of liver support, hepatocyte isolation and cell imaging via MRI he is (co-)author of 5 papers in peer reviewed journals (with more to come)!
Nathanael Raschzok successfully defended his medical doctoral thesis "summa cum laude". After three years of extremely fruitful research and development in the field of hepatocyte transplantation, cell labeling, and MR imaging of transplanted cells he is first author of three papers in peer reviewed journals (with more to come...). He currently is finishing his in vivo MRI studies of MPIO labeled transplanted hepatocytes. Transplantation of primary human hepatocytes is a promising approach in certain liver diseases. For visualisation of hepatocytes during and following cell application and the ability of a timely response to potential complications, a non-invasive modality for imaging of the transplanted cells has to be established. The aim of his studies was to label primary human hepatocytes with micron-sized iron oxide particles (MPIOs), enabling the detection of cells by clinical magnetic resonance imaging (MRI). The feasibility of preparing MPIO-labelled primary human hepatocytes detectable by clinical MR equipment was shown in vitro. MPIO-labelled cells could serve for basic research and quality control in the clinical setting of human hepatocyte transplantation. He also investigated techniques for evaluation of the particle uptake via continuum source atomic absorption spectrometry (CSAAS). Porcine liver cells were labelled with MPIOs and the iron concentration of the cell samples was investigated by a CSAAS spectrometer equipped with a Perkin-Elmer THGA graphite furnace. CSAAS enabled rapid quantification of particle load from small quantities of cells without extensive preparation steps. CSAAS could be used for quality control in a clinical setting of cell transplantation.
The Steering Committee of the Berlin-Brandenburg Center for Regenerative Therapies (BCRT) decided to involve Igor Sauer and his group as an Associated Investigator (AI). The BCRT is a cooperative research institution of the Charite University Hospital in Berlin and Germany's largest research association, the Helmholtz Association. BCRT also receives generous financial support from the BMBF and the states of Berlin and Brandenburg, as well as from the Technology Foundations in Berlin and Brandenburg, the Future Fund Berlin and from various industry partners. More than 15 regional partners from science and industry are active members of the consortium at the BCRT.