miRNA as potential biomarkers after liver transplantation: A systematic review
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The publication "miRNA as potential biomarkers after liver transplantation: A systematic review" is now available online in Transplantation Reviews. Authors are Pia F. Koch, Kristina Ludwig, Felix Krenzien, Karl H. Hillebrandt, Wenzel Schöning, Johann Pratschke, Nathanael Raschzok, Igor M. Sauer, and Simon Moosburner.

Early and accurate diagnosis of acute cellular rejection (ACR) and graft complications after liver transplantation is crucial for timely intervention and improved patient outcomes, but their diagnosis rely currently on invasive biopsy sampling, thus prompting the search for non-invasive Biomarkers. MicroRNA (miRNA) have emerged as promising biomarkers in various pathological conditions, and their potential utility in diagnosing acute cellular rejection after liver transplantation has gained significant interest.

This systematic review analyzes studies exploring miRNA as biomarkers for ACR and graft dysfunction in liver transplantation (PROSPERO ID CRD42023465278). The Cochrane Collaboration tool for assessing risk of bias was employed. Population data, identified miRNA and their dynamic regulation, as well as event prediction were compared. Data extraction and quality assessment were performed independently by two reviewers.

The results highlight the potential of miRNA as specific, non-invasive biomarkers for ACR and graft dysfunction following liver transplantation. However, further research is needed to validate these findings and establish standardized diagnostic panels to incorporate them into clinical practice and explore miRNA-based therapies in the future.
AI-based intra- and postoperative measurement from stereoimages
The publication "Redefining the Laparoscopic Spatial Sense: AI-based Intra- and Postoperative Measurement from Stereoimages“ has been accepted for the 38th AAAI Conference on Artificial Intelligence and is available via https://doi.org/10.48550/arXiv.2311.09744. The publication is the result of a fruitful collaboration between Karlsruhe Institute of Technology (KIT), Fraunhofer FIT, University of Bayreuth, and Charité – Universitätsmedizin Berlin. Authors are Leopold Müller, Patrick Hemmer, Moritz Queisner, Igor Sauer, Simeon Allmendinger, Johannes Jakubik, Michael Vössing, and Niklas Kühl.

A significant challenge in image-guided surgery is the accurate measurement task of relevant structures such as vessel segments, resection margins, or bowel lengths. While this task is an essential component of many surgeries, it involves substantial human effort and is prone to inaccuracies. In this paper, we develop a novel human-AI-based method for laparoscopic measurements utilizing stereo vision that has been guided by practicing surgeons. Based on a holistic qualitative requirements analysis, this work proposes a comprehensive measurement method, which comprises state-of-the-art machine learning architectures, such as RAFT-Stereo and YOLOv8. The developed method is assessed in various realistic experimental evaluation environments. Our results outline the potential of our method achieving high accuracies in distance measurements with errors below 1 mm. Furthermore, on-surface measurements demonstrate robustness when applied in challenging environments with textureless regions. Overall, by addressing the inherent challenges of image-guided surgery, we lay the foundation for a more robust and accurate solution for intra- and postoperative measurements, enabling more precise, safe, and efficient surgical procedures.

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Priv.-Doz. Dr. med. Karl Hillebrandt
Today Karl Hillebrandt gave an exzellent inaugural lecture entitled „Die Geister, die ich rief ... – Eine kurze Geschichte der De- und Rezellularisierung“ and is now a private lecturer (Privatdozent) at the Charité – Universitätsmedizin Berlin and habilitated in the field of "Experimental Surgery".

He is being honored for his achievements in the field of tissue engineering. His postdoctoral thesis is entitled "New approaches for the characterisation of decellularised tissues and the recellularisation of vessels".

Congratulations!

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Rise of tissue- and species-specific 3D bioprinting based on decellularized extracellular matrix-derived bioinks and bioresins
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The publication "Rise of tissue- and species-specific 3D bioprinting based on decellularized extracellular matrix-derived bioinks and bioresins" is now available online in Biomaterials and Biosystems. Authors are Laura Elomaa, Ahed Almalla, Eriselda Keshi, Karl H. Hillebrandt, Igor M. Sauer, and Marie Weinhart.

Thanks to its natural complexity and functionality, decellularized extracellular matrix (dECM) serves as an excellent foundation for creating highly cell-compatible bioinks and bioresins. This enables the bioprinted cells to thrive in an environment that closely mimics their native ECM composition and offers customizable biomechanical properties. To formulate dECM bioinks and bioresins, one must first pulverize and/or solubilize the dECM into non-crosslinked fragments, which can then be chemically modified as needed. In bioprinting, the solubilized dECM-derived material is typically deposited and/or crosslinked in a layer-by-layer fashion to build 3D hydrogel structures. Since the introduction of the first liver-derived dECM-based bioinks, a wide variety of decellularized tissue have been employed in bioprinting, including kidney, heart, cartilage, and adipose tissue among others. This review aims to summarize the critical steps involved in tissue-derived dECM bioprinting, starting from the decellularization of the ECM to the standardized formulation of bioinks and bioresins, ultimately leading to the reproducible bioprinting of tissue constructs.
New DFG research group FOR 5628 with our participation
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The Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) is establishing eight new research groups. One of these groups is FOR 5628: "Multiscale magnetic resonance elastography in cancer: The mechanical niche of tumor formation and metastatic spread – towards an improved diagnosis of cancer through mechanical imaging". The speaker and initiator is Prof. Ingolf Sack.

During the development of a tumour, the tissue changes its shape, e.g., alternating between hard and fluidic states. For this, cells exert forces and are simultaneously influenced by forces. This research group is investigating which mechanical-physical processes are behind this. How do tumours and metastases develop? What makes them resistant to therapy? The team is investigating these questions using magnetic resonance elastography (MRE) – a new clinical procedure that can be used to record the mechanical properties of body tissue. The goal is to be able to better diagnose tumours.
Dr. Karl Hillebrandt and Prof. Dr. Igor Sauer are part of the research group as PI in three projects:
  • A03 Cancer cell unjamming and jamming as prerequisites for the formation of primary and metastatic tumors
  • B03 Scaffold composition and fluid pressure in recellularized hepatic and pancreatic tumors
  • C01 Multiscale mechanical properties of tumors and tumor environment – from tissue specimens to patients

Was are happy to be part of this exzellent team!
Moderate LMWH anticoagulation improves success rate of hind limb allotransplantation in mice
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The publication "Moderate LMWH Anticoagulation Improves Success Rate of Hind Limb Allotransplantation in Mice" is now available online in Plastic & Reconstructive Surgery-Global Open. Authors are B. Kern, M.-I. Ashraf, A. Reutzel-Selke, J. Mengwasser, D. Polenz, E. Michaels, J. Pratschke, S.G. Tullius, Ch. Witzel, and I.M. Sauer.

The mouse hind limb model represents a powerful research tool in vascularized composite tissue allotransplantation, but its applicability is limited due to poor graft survival (62%–83%). Vascular thrombosis and massive hemorrhage are the major causes for these drop-outs. We hypothesize that because of better anticoagulation effect and lower risk of thrombocytopenia, application of low molecular weight heparin (LMWH) will minimize vascular complications and enhance graft and animal survival.

Fifty allogeneic hind limb transplantations were performed (C57BL/6 to DBA/2 mice) using five different anticoagulation protocols. Bleeding and thromboembolic events were recorded macroscopically by postoperative hemorrhage and livid discoloration of the graft, respectively. Graft perfusion and survival were monitored daily by capillary-refill-time of graft toes within 2–3 seconds. Vascular congestion and tissue necrosis were examined by histological evaluation of hematoxylin-eosin-stained tissue sections.

All transplantations were technically successful. Increase in thromboembolic events and a concomitant decrease in bleeding events were observed with the decreasing concentration of heparin in the perfusion solution. Although treatment of donor and recipient with low dose of LMWH could not reduce thromboembolic events, moderate dose effectively reduced these events. Compared with the poor outcome of graft perfusion with heparin alone, additional treatment of donor and recipient with low dose of LMWH improved graft and animal survival by 18%. Interestingly, animals treated with moderate dose of LMWH demonstrated 100% graft and animal survival.
Treatment of donor and recipient mice with a moderate dose of LMWH prevents vascular complications and improves the outcome of murine hind limb transplants.
ECRT Consumable Grant - Advanced Scientists
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Nils Haep successfully applied for funding from the ECRT Consumable Grant - Advanced Scientists. In his project, Nils is investigating the function of a cysteine-type endopeptidase and described mutations in the endopeptidase in induced pluripotent stem cell derived hepatocytes and their influence on Adiponutrin induced NAFLD.

Congratulations!
Priv.-Doz. Dr. med. Simon Moosburner
Today Simon Moosburner gave his inaugural lecture on "Liver Transplantation in Germany - Opportunities and Solutions for the Future". He is now – at the age of 28 (!) – a private lecturer (Privatdozent) at the Charité – Universitätsmedizin Berlin and habilitated in the field of "Experimental Surgery".

He is being honored for his achievements in the field of extracorporeal organ perfusion and organ transplantation. His postdoctoral thesis is entitled "Challenges and solutions in adults and children after liver transplantation".

Congratulations!

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Dr. Zeynep Akbal
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We are delighted to welcome Dr. Zeynep Akbal as a new member of the team!
Before she started as a post-doctoral researcher at the Digital Surgery Lab she studied communication sciences, media sciences, philosophy, and worked on developing her interdisciplinary method around virtual reality (VR) technology. She did her doctorate in philosophy at Universität Potsdam. Her dissertation is recently published as a monograph, titled "Lived-Body Experiences in Virtual Reality. A Phenomenology of the Virtual Body.”
Her research focuses on the intersection of philosophy of perception, cognitive sciences and VR. In her recent research project “Tactile Stimulation in VR” at Max Planck Institute for Human Cognitive and Brain Sciences, she focused on the behavioral consequences of haptic feedback in a VR task.

Wellcome to the team!
science x media Tandem Program: "From Slices to Spaces"
Prof. Dr. Moritz Queisner and Frédéric Eyl (Designer and Managing Director of TheGreenEyl) successfully applied to the Stiftung Charité for funding as a "science x media tandem".
The science x media tandems are the first programme in the new funding priority "Open Life Science". With this funding priority, the Charité Foundation is working to make the life sciences in Berlin more comprehensible and accessible to a broader public and to strengthen the trustworthiness of medical professionals.

Under the title "From Slices to Spaces", the tandem of Moritz Queisner and Frédéric Eyl is implementing a science parcours in which spatially complex research data from surgery and biomedicine will be made multisensually accessible to a broad audience through new visualization techniques. Building on research work on new imaging techniques by Moritz Queisner, they employ Extended Reality techniques. Due to their unique ability to link digital objects with the real environment of the viewers, the 4D images they generate are particularly suited for representing and conveying spatial information.

This is where the tandem's project comes in: 4D images are not only interesting for researchers to understand complex research data but can also provide laypeople with a less presupposing insight into research data and processes. Frédéric Eyl's media expertise will be used to make the specific visual knowledge from research comprehensible and experiential for non-experts. The science parcours is intended to integrate as a digital extension into the architecture of the new research building, "Der Simulierte Mensch", located on the premises of Charité. The parcours will include the facade, the inter-floor airspace, and the central glass surfaces within the building as its stations. By enabling users to explore 4D research data within the architecture and investigate it using their own smartphones in an AR application, concrete practices and deployment locations of new image-based technologies become experiential and comprehensible. This project not only enhances the perception of Charité and the scientific location of Berlin but also opens up places of knowledge creation to the public, making practices and techniques of life sciences more visible.


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Eriselda Keshi and Simon Moosburner CSP fellows
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Dr. Eriselda Keshi and Dr. Simon Moosburner successfully applied for the BIH Charité Clinician Scientist Program (CSP).
The program provides a unique opportunity for young medical doc- tors to combine their clinical training with protected time for research. This structured career path fosters translation of scientific discoveries into application and strengthens the innovative capacity of academic medicine.
Participants of the CSP devote 50 percent of their working hours to research over a period of three years.

Dr. Keshi will work on "NeoPancreasPrint, a 3D printed islet hosting tissue based on biocompatible ink derived from human decellularized pancreas".
Dr. Moosburner applied with this project "Alleviation of Senescence induced Ischemia-Reperfusion in Liver Grafts of Elderly Donors [SenEx".



Congratulations!
Prof. Dr. Nathanael Raschzok
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Nathanael Raschzok | Experimental Surgery | 2007

With us in the team since he was a student, he has so far climbed all academic levels with flying colours.
In recognition of his outstanding achievements in research, teaching and the promotion of young academics, Nathanael was awarded the title of Associate Professor at the Charité – Universitätsmedizin Berlin.

Congratulations, Prof. Raschzok!
DFG | Grant for Machine Perfusion RCT
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The German Research Foundation (Deutsche Forschungsgemeinschaft – DFG) s sponsoring a multicenter randomized controlled clinical trial by Prof. Dr. Georg Lurje entitled "End-ischemic hypothermic oxygenated (HOPE) or normothermic machine perfusion (NMP) compared to conventional cold storage (CCS) in donation after brain death (DBD) liver transplantation; a prospective multicenter randomized controlled trial (HOPE-NMP)".

The purpose of this study is to test the effects of end-ischemic NMP versus end-ischemic HOPE technique in a multicentre prospective randomized controlled clinical trial (RCT) on ECD liver grafts in DBD liver-transplantation (HOPE-NMP). Two-hundred-thirteen (n = 213) human whole organ liver grafts will be submitted to either NMP (n = 85) or HOPE (n = 85) directly before implantation and going to be compared to a control-group of patients (n = 43) transplanted with static cold storage preserved ECD-allografts. Primary (surgical complications as assessed by the comprehensive complication index [CCI]) and secondary (graft- and patient survival, hospital costs, hospital stay) endpoints are going to be analysed.


Congratulations !
Work with us | PhD position
We offer a funded PhD position for a computational biologist @ Experimental Surgery, Charité – Universitätsmedizin Berlin!

Are you interested to work on cutting-edge cancer research, and investigating complex-chromosomal as well as other genomic phenomena in human carcinoma cells ?

  • You will work on complex genome analyses (single-cell analyses, whole genome analyses) to detect, annotate and re-construct circular DNA using state of the art computational tools (e.g., Amplicon architect).
  • We provide motivation and high commitment in supervision in areas around experimental oncology and surgery.
  • The position allows the applicant to pursue an academic qualification, while collaborating and networking with international experts on extrachromosomal circular DNA and pancreatic carcinogenesis.

Apply now!
Send brief cover letter and CV via email to Dr. med. Matthäus Felsenstein (matthaeus.felsenstein@charite.de)
BIH Medical Student Research Stipend for Cao Zhong Jing Jin
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Cao Zhong Jing Jin under the supervision of Dr. med. Matthäus Felsenstein successfully applied for the BIH Medical Student Research Stipend on their project “Deciphering the molecular determinants for the transformation of high-grade pancreatic duct dysplasia to invasive carcinoma by single-cell transcriptomics”. She is conducting a cutting-edge research project merging molecular data with histological information in collaboration with the BIH core facilities for single-cell genomics (Dr. Thomas Conrad) and intelligent imaging (Prof. Dr. Christian Conrad). Using modern single-cell- and spatial transcriptomics on pancreatic precursor and carcinoma samples, the project aims at defining molecular signatures that drive dysplastic cells.

Congratulations!
New DFG project "4D Imaging"
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The DFG Schwerpunktprogramm „Das Digitale Bild“ (SPP 2172) funds the new project “4D Imaging: From Image Theory to Imaging Practice” (2023-2026). Principal investigators are Prof. Dr. Kathrin Friedrich (Universität Bonn) and Prof. Dr. Moritz Queisner.

The term 4D imaging refers to a new form of digital visuality in which image, action and space are inextricably interwoven. 4D technologies capture, process and transmit information about physical space and make it computable in real time. Changes due to movements and actions become calculable in real time, making 4D images particularly important in aesthetic and operational contexts where they reconceptualize various forms of human-computer interaction. The 4D Imaging project responds to the growing need in medicine to understand, use, and design these complex imaging techniques. It transfers critical reflexive knowledge from research into clinical practices to enable surgeons to use and apply 4D Imaging techniques. Especially in surgical planning, 4D Imaging techniques may improve the understanding and accessibility of spatially complex anatomical structures. To this end, the project is developing approaches to how 4D imaging can complement and transform established topographic ("2D") imaging practices.

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Work with us | PhD position

We are hiring: 3-year #PhD position @Charité – Universitätsmedizin Berlin.
  • Join our interdisciplinary team for a PhD on new #imaging technologies at the intersection of digital health, surgery and biomedicine
  • Explore new ways to understand and/or visualize anatomical structures in #4D using extended reality #XR #digitaltransformation
  • Connect theory and practice in an interdisciplinary research group
  • Open call: open to all disciplines! Yes, that’s right – design, computer science, computer visualistics, digital health, psychology, media studies, workplace studies, game design…
  • What counts is a convincing idea for your doctoral project in the field of "4D imaging“

Sounds interesting? Apply now or reach out to Moritz Queisner (moritz.queisner@charite.de) if you have any questions.

More information:
German: https://karriere.charite.de/stellenangebote/detail/wissenschaftliche-mitarbeiterin-wissenschaftlicher-mitarbeiter-dwm-technologietransfer-chirurgie-dm27222a
English: https://karriere.charite.de/stellenangebote/detail/scientific-researcher-phd-position-dfm-dm27222b

EKFS grant for functional role and clinical relevance of ecDNA in pancreatic adenocarcinoma
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Dr. Matthäus Felsenstein successfully applied for funding from the Else Kröner Fresenius Stiftung (EKFS) for his project “The functional role and clinical relevance of extrachromosomal DNA (ecDNA) in pancreatic adenocarcinoma”. In close collaboration with the excellence group around Professor Anton Henssen, he is aiming at improved understanding of unique genomic patterns as a results of complex chromosomal rearrangements in pancreatic adenocarcinoma that could drive its aggressive behavior. He will use state-of-the art three-dimensional tissue culture to enrich for neoplastic cells from primary PDAC specimen and subsequently perform genome analyses to identify samples that harbor extrachromosomal DNA. The clinical impact of these chromosomal structures will be explored by clinical correlation analyses and therapy response in vitro.

Congratulations!
Prof. Dr. Moritz Queisner
Today Moritz Queisner received his appointment certificate for the professorship (W1) for Interdisciplinary Technology Transfer and Digitization in Surgery!
The professorship is associated with the DFG-funded Cluster of Excellence
»Matters of Activity«.

Congratulations!

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On behalf of the Dean, Vice Dean Prof. Susanne Michl awards the certificate.
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